研究领域免疫学  结合蛋白  糖蛋白  

抗体来源Rabbit

克隆类型Polyclonal

交叉反应Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, 

产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量32kDa

细胞定位细胞膜 

性    状Lyophilized or Liquid

浓    度1mg/1ml

免 疫 原KLH conjugated synthetic peptide derived from human ASGPR1

亚    型IgG

纯化方法affinity purified by Protein A

储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

产品介绍background:

ASGR is a heterooligomeric receptor that is abundantly expressed on the sinusoidal surface of the hepatic plasma membrane. It is an endocytic receptor that rapidly binds and internalizes galactose-terminated glycoproteins (asialoglycoproteins or ASGP) from the circulation. The mouse ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The aa sequence of mouse ASGPR1 ECD is 89% and 79% identical to the ASGPR1 ECD of rat and human, respectively.

 

Function:

Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.

 

Subunit:

Interacts with LASS2.

 

Subcellular Location:

Membrane; Single-pass type II membrane protein.

 

Tissue Specificity:

Expressed exclusively in hepatic parenchymal cells.

 

Post-translational modifications:

Phosphorylated on a cytoplasmic Ser residue.

 

Similarity:

Contains 1 C-type lectin domain.

 

Database links:

UniProtKB/Swiss-Prot: P07306.2 human 

UniProtKB/Swiss-Prot: P34927.4 mouse

 

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.